8V54

Engaged conformation of the human mitochondrial DNA polymerase gamma bound to DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA polymerase subunit gamma-1	polymer	1229	138723.4	1	UniProt (P54098) Pfam (PF18136) In PDB	Homo sapiens (human)
2	B, C	DNA polymerase subunit gamma-2, mitochondrial	polymer	474	53787.3	2	UniProt (Q9UHN1) Pfam (PF03129) In PDB	Homo sapiens (human)	DNA polymerase gamma accessory 55 kDa subunit,p55,Mitochondrial DNA polymerase accessory subunit,MtPolB,PolG-beta
3	P	DNA (25-MER)	polymer	25	7595.9	1		Homo sapiens
4	T	DNA (44-MER)	polymer	44	13597.7	1		Homo sapiens

Sequence modifications

A: 26 - 1239 (UniProt: P54098)

PDB	External Database	Details
Met 11	-	initiating methionine
Gly 12	-	expression tag
Gly 13	-	expression tag
Ser 14	-	expression tag
His 15	-	expression tag
His 16	-	expression tag
His 17	-	expression tag
His 18	-	expression tag
His 19	-	expression tag
His 20	-	expression tag
Gly 21	-	expression tag
Ser 22	-	expression tag
Arg 23	-	expression tag
Phe 24	-	expression tag
Met 25	-	expression tag
Ala 198	Asp 198	engineered mutation
Ala 200	Glu 200	engineered mutation

B, C: 26 - 485 (UniProt: Q9UHN1)

PDB	External Database	Details
Met 12	-	initiating methionine
Ala 13	-	expression tag
Ser 14	-	expression tag
Arg 15	-	expression tag
Gly 16	-	expression tag
Ser 17	-	expression tag
His 18	-	expression tag
His 19	-	expression tag
His 20	-	expression tag
His 21	-	expression tag
His 22	-	expression tag
His 23	-	expression tag
Gly 24	-	expression tag
Ala 25	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	267491.6
All*	Total formula weight	267491.6

*Water molecules are not included.