8V47

CryoEM structure of AriA-AriB complex (Form II)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, D, E, F (A, B, C, F, H)	AriA antitoxin	polymer	464	53287.1	5	UniProt (D6IC77)	Escherichia coli B185
2	C, G (D, G)	AriB	polymer	308	34749.6	2	UniProt (D6IC76)	Escherichia coli B185
3	H, I, J, K (A, B, C, H)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	4	Chemie (ATP)

Sequence modifications

A, B, C, F, H: 2 - 464 (UniProt: D6IC77)

PDB	External Database	Details
Val 1	-	expression tag
Gln 393	Glu 393	conflict

D, G: 1 - 308 (UniProt: D6IC76)

PDB	External Database	Details
Ala 90	Glu 90	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	335934.7
Non-Polymers*	Number of molecules	4
	Total formula weight	2028.7
All*	Total formula weight	337963.4

*Water molecules are not included.