8V46

CryoEM structure of AriA-AriB complex (Form I)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, F	AriA antitoxin	polymer	464	53287.1	4	UniProt (D6IC77) In PDB	Escherichia coli B185
2	D	AriB	polymer	308	34749.6	1	UniProt (D6IC76) In PDB	Escherichia coli B185
3	A, B	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (ATP)
4	B	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

A, B, C, F: 2 - 464 (UniProt: D6IC77)

PDB	External Database	Details
Val 1	-	expression tag
Gln 393	Glu 393	engineered mutation

D: 1 - 308 (UniProt: D6IC76)

PDB	External Database	Details
Ala 90	Glu 90	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	247898.0
Non-Polymers*	Number of molecules	3
	Total formula weight	1038.7
All*	Total formula weight	248936.7

*Water molecules are not included.