8V3J

Structure-Based Engineering of a Highly Immunogenic, Conformationally Stabilized FimH Antigen for a Urinary Tract Infection Vaccine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Type 1 fimbrin D-mannose specific adhesin,Protein FimG	polymer	310	32118.6	4	UniProt (P08191) UniProt (P08190) Pfam (PF09160) Pfam (PF00419)	Escherichia coli	Protein FimH
2	E, F, G, H (A, B, C, D)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	4	Chemie (NAG)
3	I, J, K, L (A, B, C, D)	water	water		18.0	857	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 279 (UniProt: P08191)

PDB	External Database	Details
Ser 7	Asn 28	engineered mutation
Ala 15	Gly 36	engineered mutation
Ala 16	Gly 37	engineered mutation
Ala 27	Val 48	engineered mutation
Ser 70	Asn 91	engineered mutation
Gln 228	Asn 249	engineered mutation
Gly 280	-	linker
Gly 281	-	linker
Ser 282	-	linker
Ser 283	-	linker
Gly 284	-	linker
Gly 285	-	linker
Gly 286	-	linker

A, B, C, D: 287 - 300 (UniProt: P08190)

PDB	External Database	Details
Gly 301	-	expression tag
Gly 302	-	expression tag
His 303	-	expression tag
His 304	-	expression tag
His 305	-	expression tag
His 306	-	expression tag
His 307	-	expression tag
His 308	-	expression tag
His 309	-	expression tag
His 310	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	128474.4
Non-Polymers*	Number of molecules	4
	Total formula weight	884.8
All*	Total formula weight	129359.2

*Water molecules are not included.