8V26

H2BK120ub-modified nucleosome ubiquitin position 2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E	Histone H3.2	polymer	136	15435.1	2	UniProt (P84233) Pfam (PF00125) In PDB	Xenopus laevis (African clawed frog)	Histone H3
2	B, F	Histone H4	polymer	103	11394.4	2	UniProt (A0A8J1LTD2) Pfam (PF15511) In PDB	Xenopus laevis (African clawed frog)
3	C, G	Histone H2A	polymer	130	14109.4	2	UniProt (Q6AZJ8) Pfam (PF00125) Pfam (PF16211) In PDB	Xenopus laevis (African clawed frog)
4	D, H	Histone H2B 1.1	polymer	123	13629.9	2	UniProt (P02281) Pfam (PF00125) In PDB	Xenopus laevis (African clawed frog)	H2B1.1
5	I	601 DNA (147-MER)	polymer	147	45138.8	1		synthetic construct
6	J	601 DNA (147-MER)	polymer	147	45610.0	1		synthetic construct
7	K	Ubiquitin	polymer	80	9036.4	1	UniProt (P0CG47) Pfam (PF00240) In PDB	Homo sapiens (human)

Sequence modifications

A, E: 0 - 135 (UniProt: P84233)

PDB	External Database	Details
Ala 102	Gly 103	engineered mutation

D, H: 1 - 122 (UniProt: P02281)

PDB	External Database	Details
Met 0	-	expression tag
Thr 29	Ser 33	engineered mutation
Cys 117	Lys 121	engineered mutation

K: 1 - 76 (UniProt: P0CG47)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag
Cys 76	Gly 76	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	11
	Total formula weight	208923.0
All*	Total formula weight	208923.0

*Water molecules are not included.