8V1U
Human DNA Ligase I F872A bound to adenylated nicked DNA with a 5' terminal ribonucleotide
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA ligase 1 | polymer | 647 | 71956.2 | 1 | UniProt (P18858) Pfam (PF04675) Pfam (PF01068) Pfam (PF04679) | Homo sapiens (human) | DNA ligase I,Polydeoxyribonucleotide synthase [ATP] 1 |
| 2 | B (B) | DNA (5'-D(*GP*CP*TP*GP*AP*TP*GP*CP*GP*TP*C)-3') | polymer | 11 | 3365.2 | 1 | synthetic construct | ||
| 3 | C (C) | DNA/RNA (5'-R(P*G)-D(P*TP*CP*GP*GP*AP*C)-3') | polymer | 7 | 2154.4 | 1 | synthetic construct | ||
| 4 | D (D) | DNA (5'-D(*GP*TP*CP*CP*GP*AP*CP*GP*AP*CP*GP*CP*AP*TP*CP*AP*GP*C)-3') | polymer | 18 | 5486.6 | 1 | synthetic construct | ||
| 5 | E (A) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 1 | Chemie (PEG) PubChem (8117) | |||
| 6 | F, G (A) | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID | non-polymer | 195.2 | 2 | Chemie (MES) PubChem (4478249) | |||
| 7 | H (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) PubChem (312) | |||
| 8 | I (B) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) PubChem (753) | |||
| 9 | J, L (B, C) | SODIUM ION | non-polymer | 23.0 | 2 | Chemie (NA) PubChem (923) | |||
| 10 | K (C) | ADENOSINE MONOPHOSPHATE | non-polymer | 347.2 | 1 | Chemie (AMP) PubChem (224) PubChem (34768) PubChem (83862) PubChem (446048) PubChem (515384) PubChem (638042) PubChem (5316444) PubChem (5742864) PubChem (12163610) PubChem (12358355) PubChem (16397049) PubChem (16760266) PubChem (40467049) PubChem (40469468) PubChem (44348067) PubChem (45358029) PubChem (46874558) PubChem (40459109) PubChem (46875232) PubChem (177678969) PubChem (6083) PubChem (1549371) PubChem (92144436) PubChem (57358045) PubChem (57879893) PubChem (57879902) PubChem (12358354) PubChem (122197642) PubChem (162290139) PubChem (162946266) PubChem (171699505) | |||
| 11 | M, N, O, P (A, B, C, D) | water | water | 18.0 | 469 | Chemie (HOH) |
Sequence modifications
A: 262 - 904 (UniProt: P18858)
| PDB | External Database | Details |
|---|---|---|
| Ser 258 | - | expression tag |
| Asn 259 | - | expression tag |
| Asp 260 | - | expression tag |
| Met 261 | - | expression tag |
| Ala 872 | Phe 872 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 82962.4 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 1017.3 | |
| All* | Total formula weight | 83979.7 |
