8V1Q

Herpes simplex virus 1 polymerase holoenzyme bound to DNA in both open/closed conformations

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA polymerase	polymer	1199	133614.3	1	UniProt (H9E937) In PDB	Human alphaherpesvirus 1 strain KOS
2	B	DNA polymerase processivity factor	polymer	349	37275.1	1	UniProt (A0A181ZFK4) In PDB	Human alphaherpesvirus 1 strain KOS
3	P	Primer DNA	polymer	32	9866.4	1		synthetic construct
4	T	template DNA	polymer	50	15223.8	1		synthetic construct
5	A	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6		water	water		18.0	3	Chemie (HOH)

Sequence modifications

A: 43 - 1235 (UniProt: H9E937)

PDB	External Database	Details
His 37	-	expression tag
His 38	-	expression tag
His 39	-	expression tag
His 40	-	expression tag
His 41	-	expression tag
His 42	-	expression tag

B: 2 - 340 (UniProt: A0A181ZFK4)

PDB	External Database	Details
Gly -8	-	expression tag
Pro -7	-	expression tag
Ile -6	-	expression tag
Ser -5	-	expression tag
Glu -4	-	expression tag
Phe -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Arg 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	195979.6
Non-Polymers*	Number of molecules	2
	Total formula weight	48.6
All*	Total formula weight	196028.2

*Water molecules are not included.