8V10

Structure of a Saccharomyces cerevisiae Mps1 peptide bound to dwarf Ndc80 Complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Kinetochore protein NDC80	polymer	279	33256.9	1	UniProt (P40460) Pfam (PF03801) Pfam (PF18077) In PDB	Saccharomyces cerevisiae (brewer's yeast)
2	B	Mps1/NUF2 chimera protein	polymer	233	27375.2	1	UniProt (P54199) UniProt (P33895) Pfam (PF03800) In PDB	Saccharomyces cerevisiae (brewer's yeast)
3	C	Kinetochore protein SPC24	polymer	100	11881.5	1	UniProt (Q04477) Pfam (PF08286) In PDB	Saccharomyces cerevisiae (brewer's yeast)
4	D	Kinetochore protein SPC25	polymer	115	12822.6	1	UniProt (P40014) In PDB	Saccharomyces cerevisiae (brewer's yeast)
5	A	NICKEL (II) ION	non-polymer		58.7	1	Chemie (NI)

Sequence modifications

A: 114 - 318 (UniProt: P40460)

PDB	External Database	Details
Ser 111	-	expression tag
Asn 112	-	expression tag
Ala 113	-	expression tag

A: 621 - 691 (UniProt: P40460)
B: 137 - 171 (UniProt: P54199)

PDB	External Database	Details
Met 136	-	initiating methionine

B: 1002 - 1153 (UniProt: P33895)
B: 1407 - 1451 (UniProt: P33895)
C: 1 - 48 (UniProt: Q04477)
C: 49 - 100 (UniProt: Q04477)
D: 1 - 31 (UniProt: P40014)
D: 32 - 115 (UniProt: P40014)

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	85336.2
Non-Polymers*	Number of molecules	1
	Total formula weight	58.7
All*	Total formula weight	85394.9

*Water molecules are not included.