8UTD
CryoEM Structure of HCA2-Gi1 in complex with MK-1903
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (R) | Hydroxycarboxylic acid receptor 2 | polymer | 368 | 42297.9 | 1 | UniProt (Q8TDS4) Pfam (PF00001) | Homo sapiens (human) | |
| 2 | B (D) | scFv16 | polymer | 267 | 28668.9 | 1 | Lama glama | ||
| 3 | C (A) | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 354 | 40445.1 | 1 | UniProt (P63096) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein |
| 4 | D (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 358 | 39418.1 | 1 | UniProt (P62873) Pfam (PF25391) | Homo sapiens (human) | Transducin beta chain 1 |
| 5 | E (C) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 6 | F (R) | (4aR,5aR)-4,4a,5,5a-tetrahydro-1H-cyclopropa[4,5]cyclopenta[1,2-c]pyrazole-3-carboxylic acid | non-polymer | 164.2 | 1 | Chemie (XOT) |
Sequence modifications
R: 1 - 363 (UniProt: Q8TDS4)
A: 1 - 354 (UniProt: P63096)
B: 2 - 340 (UniProt: P62873)
| PDB | External Database | Details |
|---|---|---|
| Gln -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Thr -1 | - | expression tag |
| Thr 0 | - | expression tag |
| Ala 124 | Asp 124 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ala 203 | Gly 203 | engineered mutation |
| Ser 326 | Ala 326 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -17 | - | expression tag |
| His -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| Leu -10 | - | expression tag |
| Glu -9 | - | expression tag |
| Val -8 | - | expression tag |
| Leu -7 | - | expression tag |
| Phe -6 | - | expression tag |
| Gln -5 | - | expression tag |
| Gly -4 | - | expression tag |
| Pro -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Gly 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 158691.1 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 164.2 | |
| All* | Total formula weight | 158855.2 |
