8UR1
Crystal structure N-acetylneuraminate lyase (NanA) from Klebsiella aerogenes (pyruvate bound halide free active site)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | N-acetylneuraminate lyase | polymer | 305 | 33738.6 | 1 | UniProt (A0A0H3FJT8) Pfam (PF00701) | Klebsiella aerogenes KCTC 2190 | |
| 2 | B, C, D, E (A) | SODIUM ION | non-polymer | 23.0 | 4 | Chemie (NA) | |||
| 3 | F, G, H, I (A) | CHLORIDE ION | non-polymer | 35.5 | 4 | Chemie (CL) | |||
| 4 | J, K, L (A) | SULFATE ION | non-polymer | 96.1 | 3 | Chemie (SO4) | |||
| 5 | M, N, O, P (A) | GLYCEROL | non-polymer | 92.1 | 4 | Chemie (GOL) | |||
| 6 | Q, R (A) | NITRATE ION | non-polymer | 62.0 | 2 | Chemie (NO3) | |||
| 7 | S (A) | PENTAETHYLENE GLYCOL | non-polymer | 238.3 | 1 | Chemie (1PE) | |||
| 8 | T (A) | water | water | 18.0 | 173 | Chemie (HOH) |
Sequence modifications
A: 1 - 297 (UniProt: A0A0H3FJT8)
| PDB | External Database | Details |
|---|---|---|
| Met -7 | - | initiating methionine |
| Ala -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| His -2 | - | expression tag |
| His -1 | - | expression tag |
| His 0 | - | expression tag |
| Ala 93 | Val 93 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 33738.6 | |
| Non-Polymers* | Number of molecules | 18 |
| Total formula weight | 1252.6 | |
| All* | Total formula weight | 34991.2 |
