8UQ8

Crystal structure of RNF168 (RING)-UbcH5c fused to H2A-H2B via a 2-residue linker

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, a)	E3 ubiquitin-protein ligase RNF168,Ubiquitin-conjugating enzyme E2 D3,Histone H2B type 2-E,Histone H2A type 1-B/E	polymer	437	48807.2	2	UniProt (Q8IYW5) UniProt (P61077) UniProt (Q16778) UniProt (P04908) Pfam (PF14447) Pfam (PF00179) Pfam (PF00125) Pfam (PF16211)	Homo sapiens (human)
2	C, CA, D, DA, E... (A, a)	CHLORIDE ION	non-polymer		35.5	21	Chemie (CL) PubChem (312)
3	G, H, L, Y, Z (A, a)	GLYCEROL	non-polymer		92.1	5	Chemie (GOL) PubChem (753)
4	AA, BA, I, J (a, A)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
5	GA, HA, K, M, N (a, A)	SODIUM ION	non-polymer		23.0	5	Chemie (NA) PubChem (923)
6	LA, MA (A, a)	water	water		18.0	192	Chemie (HOH)

Sequence modifications

A, a: 1 - 94 (UniProt: Q8IYW5)

PDB	External Database	Details
Gly -1	-	expression tag
His 0	-	expression tag
Ser 95	-	linker
Gly 96	-	linker
Ser 97	-	linker
Gly 98	-	linker
Ser 99	-	linker
Gly 100	-	linker
Ser 101	-	linker

A, a: 1002 - 1147 (UniProt: P61077)

PDB	External Database	Details
Gly 1148	-	linker
Ser 1149	-	linker

A, a: 2033 - 2123 (UniProt: Q16778)

PDB	External Database	Details
Ser 3011	-	linker

A, a: 3012 - 3105 (UniProt: P04908)

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	97614.4
Non-Polymers*	Number of molecules	35
	Total formula weight	1581.6
All*	Total formula weight	99196.0

*Water molecules are not included.