8UP8

Structure of atypical asparaginase from Rhodospirillum rubrum (mutant Y21F, complex with L-Asp)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Asparaginase	polymer	185	19785.5	4	UniProt (Q2RMX1) Pfam (PF00710)	Rhodospirillum rubrum ATCC 11170
2	E, F, J (A, B, C)	ASPARTIC ACID	non-polymer		133.1	3	Chemie (ASP)
3	G, K (B, D)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
4	H, I, L, M (B, D)	TETRAETHYLENE GLYCOL	non-polymer		194.2	4	Chemie (PG4)
5	N, O, P, Q (A, B, C, D)	water	water		18.0	382	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 172 (UniProt: Q2RMX1)

PDB	External Database	Details
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Ser 0	-	expression tag
Phe 21	Tyr 21	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	79142.0
Non-Polymers*	Number of molecules	9
	Total formula weight	1247.1
All*	Total formula weight	80389.1

*Water molecules are not included.