8UP6

Structure of atypical asparaginase from Rhodospirillum rubrum (mutant K19A) in complex with L-Asp

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Asparaginase	polymer	185	19743.4	4	UniProt (Q2RMX1) Pfam (PF00710)	Rhodospirillum rubrum ATCC 11170
2	E, H (B, C)	ASPARTIC ACID	non-polymer		133.1	2	Chemie (ASP)
3	F, G, I (B, D)	TETRAETHYLENE GLYCOL	non-polymer		194.2	3	Chemie (PG4)
4	J, K, L, M (A, B, C, D)	water	water		18.0	384	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 172 (UniProt: Q2RMX1)

PDB	External Database	Details
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Ser 0	-	expression tag
Ala 19	Lys 19	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	78973.6
Non-Polymers*	Number of molecules	5
	Total formula weight	848.9
All*	Total formula weight	79822.5

*Water molecules are not included.