8UP5

Structure of the KEOPS complex (Cgi121/Bud32/Kae1/Pcc1) bound to tRNA in its native-like conformation.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (T)	tRNA	polymer	77	24758.7	1	GenBank (6626255)	Methanocaldococcus jannaschii
2	B (B)	Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein	polymer	203	23957.5	1	UniProt (Q58530) Pfam (PF01163)	Methanocaldococcus jannaschii
3	C (K)	Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein	polymer	325	35869.4	1	UniProt (Q58530) Pfam (PF00814)	Methanocaldococcus jannaschii
4	D (C)	Regulatory protein Cgi121	polymer	148	16963.9	1	UniProt (A0A832SJR9) Pfam (PF08617) UniProt (by SIFTS) (Q57646)	Methanocaldococcus jannaschii
5	E (P)	KEOPS complex subunit Pcc1	polymer	175	19731.8	1	UniProt (Q8TZI1) Pfam (PF09341)	Pyrococcus furiosus DSM 3638

Sequence modifications

T: 0 - 76 (GenBank: 6626255)

PDB	External Database	Details
C 75	U 863891	conflict

B: 333 - 535 (UniProt: Q58530)

PDB	External Database	Details
Arg 478	Glu 478	engineered mutation

K: 1 - 324 (UniProt: Q58530)

PDB	External Database	Details
Pro 0	-	expression tag

C: 6 - 150 (UniProt: A0A832SJR9)

PDB	External Database	Details
Met 3	-	expression tag
Asp 4	-	expression tag
Pro 5	-	expression tag

P: 94 - 175 (UniProt: Q8TZI1)

PDB	External Database	Details
Gly 83	-	linker
Ser 84	-	linker
Gly 85	-	linker
Ser 86	-	linker
Gly 87	-	linker
Ser 88	-	linker
Gly 89	-	linker
Ser 90	-	linker
Gly 91	-	linker
Ser 92	-	linker
Gly 93	-	linker
Tyr 168	Ala 75	engineered mutation
Arg 172	Val 79	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	121281.3
All*	Total formula weight	121281.3

*Water molecules are not included.