8UP3

Structure of atypical asparaginase from Rhodospirillum rubrum (mutant Y21F)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	Asparaginase	polymer	185	19755.5	8	UniProt (Q2RMX1) Pfam (PF00710)	Rhodospirillum rubrum ATCC 11170
2	BA, CA, DA, EA, FA... (D, A, E, F, G...)	1,2-ETHANEDIOL	non-polymer		62.1	25	Chemie (EDO)
3	AA, GA, KA, NA, Q... (C, D, E, F, A...)	CHLORIDE ION	non-polymer		35.5	11	Chemie (CL)
4	HA, LA (E, F)	ASPARTIC ACID	non-polymer		133.1	2	Chemie (ASP)
5	AB, BB, UA, VA, WA... (G, H, A, B, C...)	water	water		18.0	1076	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F, G, H: 1 - 172 (UniProt: Q2RMX1)

PDB	External Database	Details
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag
Phe 21	Tyr 21	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	158043.8
Non-Polymers*	Number of molecules	38
	Total formula weight	2207.9
All*	Total formula weight	160251.6

*Water molecules are not included.