8UNW
CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class L)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 380 | 44326.2 | 1 | UniProt (P63092) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-stimulating G alpha protein |
| 2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 343 | 37574.0 | 1 | UniProt (P62873) Pfam (PF25391) | Homo sapiens (human) | |
| 3 | C (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 4 | D (R) | Beta-2 adrenergic receptor | polymer | 460 | 51767.2 | 1 | UniProt (P07550) Pfam (PF00001) | Homo sapiens (human) | Beta-2 adrenoreceptor,Beta-2 adrenoceptor |
| 5 | E (A) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466) | |||
| 6 | F (R) | (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol | non-polymer | 209.2 | 1 | Chemie (G1I) PubChem (12353636) PubChem (12353638) PubChem (138714551) | |||
| 7 | G (R) | water | water | 18.0 | 1 | Chemie (HOH) |
Sequence modifications
B: 2 - 340 (UniProt: P62873)
R: -6 - 421 (UniProt: P07550)
| PDB | External Database | Details |
|---|---|---|
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Gly 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met -30 | - | expression tag |
| Lys -29 | - | expression tag |
| Thr -28 | - | expression tag |
| Ile -27 | - | expression tag |
| Ile -26 | - | expression tag |
| Ala -25 | - | expression tag |
| Leu -24 | - | expression tag |
| Ser -23 | - | expression tag |
| Tyr -22 | - | expression tag |
| Ile -21 | - | expression tag |
| Phe -20 | - | expression tag |
| Cys -19 | - | expression tag |
| Leu -18 | - | expression tag |
| Val -17 | - | expression tag |
| Phe -16 | - | expression tag |
| Ala -15 | - | expression tag |
| Asp -14 | - | expression tag |
| Tyr -13 | - | expression tag |
| Lys -12 | - | expression tag |
| Asp -11 | - | expression tag |
| Asp -10 | - | expression tag |
| Asp -9 | - | expression tag |
| Asp -8 | - | expression tag |
| Ala -7 | - | expression tag |
| Arg 9 | Gly 16 | variant |
| Glu 18 | - | insertion |
| Asn 19 | - | insertion |
| Leu 20 | - | insertion |
| Tyr 21 | - | insertion |
| Phe 22 | - | insertion |
| Gln 23 | - | insertion |
| Gly 24 | - | insertion |
| Gln 27 | Glu 27 | variant |
| Thr 96 | Met 96 | conflict |
| Thr 98 | Met 98 | conflict |
| Glu 187 | Asn 187 | conflict |
| Leu 366 | - | insertion |
| Glu 367 | - | insertion |
| Val 368 | - | insertion |
| Leu 369 | - | insertion |
| Phe 370 | - | insertion |
| Gln 371 | - | insertion |
| Gly 372 | - | insertion |
| Pro 373 | - | insertion |
| Ala 386 | Cys 378 | conflict |
| Ala 414 | Cys 406 | conflict |
| Glu 422 | - | expression tag |
| Thr 423 | - | expression tag |
| Ser 424 | - | expression tag |
| Gln 425 | - | expression tag |
| Val 426 | - | expression tag |
| Ala 427 | - | expression tag |
| Pro 428 | - | expression tag |
| Ala 429 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 141528.5 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 732.4 | |
| All* | Total formula weight | 142261.0 |
