8UNP
CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class E)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 380 | 44326.2 | 1 | UniProt (P63092) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-stimulating G alpha protein |
| 2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 343 | 37574.0 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (human) | |
| 3 | C (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 4 | D (R) | Beta-2 adrenergic receptor | polymer | 460 | 51767.2 | 1 | UniProt (P07550) Pfam (PF00001) | Homo sapiens (human) | Beta-2 adrenoreceptor,Beta-2 adrenoceptor |
| 5 | E (A) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) | |||
| 6 | F (R) | (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol | non-polymer | 209.2 | 1 | Chemie (G1I) | |||
| 7 | G (R) | water | water | 18.0 | 1 | Chemie (HOH) |
Sequence modifications
B: 2 - 340 (UniProt: P62873)
R: -6 - 421 (UniProt: P07550)
| PDB | External Database | Details |
|---|---|---|
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Gly 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met -30 | - | expression tag |
| Lys -29 | - | expression tag |
| Thr -28 | - | expression tag |
| Ile -27 | - | expression tag |
| Ile -26 | - | expression tag |
| Ala -25 | - | expression tag |
| Leu -24 | - | expression tag |
| Ser -23 | - | expression tag |
| Tyr -22 | - | expression tag |
| Ile -21 | - | expression tag |
| Phe -20 | - | expression tag |
| Cys -19 | - | expression tag |
| Leu -18 | - | expression tag |
| Val -17 | - | expression tag |
| Phe -16 | - | expression tag |
| Ala -15 | - | expression tag |
| Asp -14 | - | expression tag |
| Tyr -13 | - | expression tag |
| Lys -12 | - | expression tag |
| Asp -11 | - | expression tag |
| Asp -10 | - | expression tag |
| Asp -9 | - | expression tag |
| Asp -8 | - | expression tag |
| Ala -7 | - | expression tag |
| Arg 9 | Gly 16 | variant |
| Glu 18 | - | insertion |
| Asn 19 | - | insertion |
| Leu 20 | - | insertion |
| Tyr 21 | - | insertion |
| Phe 22 | - | insertion |
| Gln 23 | - | insertion |
| Gly 24 | - | insertion |
| Gln 27 | Glu 27 | variant |
| Thr 96 | Met 96 | conflict |
| Thr 98 | Met 98 | conflict |
| Glu 187 | Asn 187 | conflict |
| Leu 366 | - | insertion |
| Glu 367 | - | insertion |
| Val 368 | - | insertion |
| Leu 369 | - | insertion |
| Phe 370 | - | insertion |
| Gln 371 | - | insertion |
| Gly 372 | - | insertion |
| Pro 373 | - | insertion |
| Ala 386 | Cys 378 | conflict |
| Ala 414 | Cys 406 | conflict |
| Glu 422 | - | expression tag |
| Thr 423 | - | expression tag |
| Ser 424 | - | expression tag |
| Gln 425 | - | expression tag |
| Val 426 | - | expression tag |
| Ala 427 | - | expression tag |
| Pro 428 | - | expression tag |
| Ala 429 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 141528.5 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 732.4 | |
| All* | Total formula weight | 142261.0 |
