8ULT

Cryo-EM structure of the BG505 SOSIPv2 in complex with bNAb 04_A06 Fabs

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E (A, C, E)	Envelope glycoprotein gp120	polymer	479	53864.1	3	UniProt (Q2N0S6)	Human immunodeficiency virus 1	Env polyprotein
2	B, D, F (B, D, F)	Envelope glycoprotein gp41	polymer	153	17146.5	3	UniProt (Q2N0S5) UniProt (by SIFTS) (D0PUM0)	Human immunodeficiency virus 1
3	G, H, I (H, I, J)	04_A06 Fab Heavy Chain	polymer	245	26690.9	3		Homo sapiens
4	J, K, L (L, M, N)	04_A06 Fab Light Chain	polymer	211	23142.6	3		Homo sapiens
5	DA, M, S (d, G, S)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	3	In PDB GlyTouCan (G15407YE)
6	AA, BA, CA, EA, N... (a, b, c, e, K...)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	16	In PDB GlyTouCan (G42666HT)
7	AB, BB, CB, DB, EB... (E, A, C)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	29	Chemie (NAG)

Sequence modifications

A, C, E: 33 - 504 (UniProt: Q2N0S6)

PDB	External Database	Details
Asn 332	Thr 330	conflict
Cys 501	Ala 498	conflict
Val 505	-	expression tag
Val 506	-	expression tag
Gly 507	-	expression tag
Arg 508	-	expression tag
Arg 509	-	expression tag
Arg 510	-	expression tag
Arg 511	-	expression tag
Arg 512	-	expression tag
Arg 513	-	expression tag

B, D, F: 512 - 664 (UniProt: Q2N0S5)

PDB	External Database	Details
Pro 559	Ile 556	engineered mutation
Cys 605	Thr 602	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	362532.3
Branched	Number of molecules	19
	Total formula weight	8550.0
Non-Polymers*	Number of molecules	29
	Total formula weight	6415.0
All*	Total formula weight	377497.3

*Water molecules are not included.