8UKZ

Structure of P450Blt from Micromonospora sp. MW-13 with E238A Mutation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C	Cytochrome P450-SU1	polymer	420	45274.2	3	UniProt (A0A3E2YLT4) Pfam (PF00067) In PDB	Micromonospora sp. MW-13
2	A, B, C	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	3	Chemie (HEM)
3	A, B, C	trifluoroacetic acid	non-polymer		114.0	4	Chemie (TFA)
4	A, B, C	ACETATE ION	non-polymer		59.0	4	Chemie (ACT)
5	B	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
6		water	water		18.0	212	Chemie (HOH)

Sequence modifications

A, B, C: 1 - 399 (UniProt: A0A3E2YLT4)

PDB	External Database	Details
Met -20	-	initiating methionine
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Val -6	-	expression tag
Pro -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag
Ala 238	Glu 238	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	135822.7
Non-Polymers*	Number of molecules	12
	Total formula weight	2647.8
All*	Total formula weight	138470.6

*Water molecules are not included.