8UI0

Structure of poised transcription complex Pol II-DSIF-NELF - pre-translocated

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	X	Negative elongation factor E	polymer	380	43320.9	1	UniProt (P18615) Pfam (PF00076) In PDB	Homo sapiens (human)	NELF-E,RNA-binding protein RD
2	V	Negative elongation factor B	polymer	554	63233.3	1	UniProt (Q8WX92) Pfam (PF06209) In PDB	Homo sapiens (human)	NELF-B,Cofactor of BRCA1
3	W	Negative elongation factor C/D	polymer	590	66315.4	1	UniProt (Q8IXH7) Pfam (PF04858) In PDB	Homo sapiens (human)	NELF-C/D,TH1-like protein
4	A	DNA-directed RNA polymerase subunit	polymer	1970	217450.1	1	UniProt (A0A8D1DPV6) Pfam (PF04997) Pfam (PF05001) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) Pfam (PF04992) Pfam (PF04990) In PDB	Sus scrofa (pig)
5	B	DNA-directed RNA polymerase subunit beta	polymer	1174	134041.4	1	UniProt (A0A4X1TVZ5) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF04566) Pfam (PF04567) Pfam (PF00562) Pfam (PF04560) UniProt (by SIFTS) (I3LGP4) In PDB	Sus scrofa (pig)
6	C	DNA-directed RNA polymerase II subunit RPB3	polymer	271	30997.6	1	UniProt (A0A481DF93) Pfam (PF01193) Pfam (PF01000) UniProt (by SIFTS) (I3LCH3) In PDB	Sus scrofa (pig)
7	D	RNA polymerase Rpb4/RPC9 core domain-containing protein	polymer	142	16331.3	1	UniProt (A0A8D0KES4) Pfam (PF03874) UniProt (by SIFTS) (A0A4X1VM56) In PDB	Sus scrofa (pig)
8	E	DNA-directed RNA polymerase II subunit E	polymer	210	24644.3	1	UniProt (A0A4X1VTX4) Pfam (PF03871) Pfam (PF01191) UniProt (by SIFTS) (I3LSI7) In PDB	Sus scrofa (pig)	RPB5
9	F	DNA-directed RNA polymerases I, II, and III subunit RPABC2	polymer	127	14477.0	1	UniProt (A0A4X1VEK9) Pfam (PF01192) In PDB	Sus scrofa (pig)	DNA-directed RNA polymerase II subunit F,RPB6 homolog
10	G	DNA-directed RNA polymerase subunit	polymer	171	19227.2	1	UniProt (A0A4X1VKG7) Pfam (PF03876) Pfam (PF00575) In PDB	Sus scrofa (pig)
11	H	DNA-directed RNA polymerases I, II, and III subunit RPABC3	polymer	150	17162.3	1	UniProt (A0A8D1AQR7) Pfam (PF03870) UniProt (by SIFTS) (I3LCB2) In PDB	Sus scrofa (pig)
12	I	DNA-directed RNA polymerase II subunit RPB9	polymer	125	14541.2	1	UniProt (P60899) Pfam (PF02150) Pfam (PF01096) In PDB	Sus scrofa (pig)	RNA polymerase II subunit B9,DNA-directed RNA polymerase II subunit I,RNA polymerase II 14.5 kDa subunit,RPB14.5
13	J	DNA-directed RNA polymerases I, II, and III subunit RPABC5	polymer	67	7655.1	1	UniProt (A0A8W4F9W9) Pfam (PF01194) In PDB	Sus scrofa (pig)
14	K	DNA-directed RNA polymerase II subunit RPB11-a	polymer	115	13068.0	1	UniProt (F1RKE4) Pfam (PF13656) In PDB	Sus scrofa (pig)
15	L	RNA polymerase II subunit K	polymer	58	7018.2	1	UniProt (A0A4X1TRS6) Pfam (PF03604) In PDB	Sus scrofa (pig)
16	N	DNA	polymer	50	15409.9	1		synthetic construct
17	P	RNA	polymer	16	5077.0	1		synthetic construct
18	T	DNA (38-MER)	polymer	38	11728.6	1		synthetic construct
19	U	Negative elongation factor A	polymer	528	57343.6	1	UniProt (Q9H3P2) In PDB	Homo sapiens (human)	NELF-A,Wolf-Hirschhorn syndrome candidate 2 protein
20	Z	Transcription elongation factor SPT5	polymer	1087	121145.5	1	UniProt (O00267) Pfam (PF11942) Pfam (PF03439) Pfam (PF00467) In PDB	Homo sapiens (human)	hSPT5,DRB sensitivity-inducing factor 160 kDa subunit,DSIF p160,DRB sensitivity-inducing factor large subunit,DSIF large subunit,Tat-cotransactivator 1 protein,Tat-CT1 protein
21	I, J, L, A, B...	ZINC ION	non-polymer		65.4	8	Chemie (ZN)
22	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Contents of the asymmetric unit

Polymers	Number of chains	20
	Total formula weight	900187.7
Non-Polymers*	Number of molecules	9
	Total formula weight	547.6
All*	Total formula weight	900735.3

*Water molecules are not included.