8UI0
Structure of poised transcription complex Pol II-DSIF-NELF - pre-translocated
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (X) | Negative elongation factor E | polymer | 380 | 43320.9 | 1 | UniProt (P18615) Pfam (PF00076) | Homo sapiens (human) | NELF-E,RNA-binding protein RD |
| 2 | B (V) | Negative elongation factor B | polymer | 554 | 63233.3 | 1 | UniProt (Q8WX92) Pfam (PF06209) | Homo sapiens (human) | NELF-B,Cofactor of BRCA1 |
| 3 | C (W) | Negative elongation factor C/D | polymer | 590 | 66315.4 | 1 | UniProt (Q8IXH7) Pfam (PF04858) | Homo sapiens (human) | NELF-C/D,TH1-like protein |
| 4 | D (A) | DNA-directed RNA polymerase subunit | polymer | 1970 | 217450.1 | 1 | UniProt (A0A8D1DPV6) Pfam (PF04997) Pfam (PF05001) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) Pfam (PF04992) Pfam (PF04990) | Sus scrofa (pig) | |
| 5 | E (B) | DNA-directed RNA polymerase subunit beta | polymer | 1174 | 134041.4 | 1 | UniProt (A0A4X1TVZ5) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF04566) Pfam (PF04567) Pfam (PF00562) Pfam (PF04560) UniProt (by SIFTS) (I3LGP4) | Sus scrofa (pig) | |
| 6 | F (C) | DNA-directed RNA polymerase II subunit RPB3 | polymer | 271 | 30997.6 | 1 | UniProt (A0A481DF93) Pfam (PF01193) Pfam (PF01000) UniProt (by SIFTS) (I3LCH3) | Sus scrofa (pig) | |
| 7 | G (D) | RNA polymerase Rpb4/RPC9 core domain-containing protein | polymer | 142 | 16331.3 | 1 | UniProt (A0A8D0KES4) Pfam (PF03874) UniProt (by SIFTS) (A0A4X1VM56) | Sus scrofa (pig) | |
| 8 | H (E) | DNA-directed RNA polymerase II subunit E | polymer | 210 | 24644.3 | 1 | UniProt (A0A4X1VTX4) Pfam (PF03871) Pfam (PF01191) UniProt (by SIFTS) (I3LSI7) | Sus scrofa (pig) | RPB5 |
| 9 | I (F) | DNA-directed RNA polymerases I, II, and III subunit RPABC2 | polymer | 127 | 14477.0 | 1 | UniProt (A0A4X1VEK9) Pfam (PF01192) | Sus scrofa (pig) | DNA-directed RNA polymerase II subunit F,RPB6 homolog |
| 10 | J (G) | DNA-directed RNA polymerase subunit | polymer | 171 | 19227.2 | 1 | UniProt (A0A4X1VKG7) Pfam (PF03876) Pfam (PF00575) | Sus scrofa (pig) | |
| 11 | K (H) | DNA-directed RNA polymerases I, II, and III subunit RPABC3 | polymer | 150 | 17162.3 | 1 | UniProt (A0A8D1AQR7) Pfam (PF03870) UniProt (by SIFTS) (I3LCB2) | Sus scrofa (pig) | |
| 12 | L (I) | DNA-directed RNA polymerase II subunit RPB9 | polymer | 125 | 14541.2 | 1 | UniProt (P60899) Pfam (PF02150) Pfam (PF01096) | Sus scrofa (pig) | RNA polymerase II subunit B9,DNA-directed RNA polymerase II subunit I,RNA polymerase II 14.5 kDa subunit,RPB14.5 |
| 13 | M (J) | DNA-directed RNA polymerases I, II, and III subunit RPABC5 | polymer | 67 | 7655.1 | 1 | UniProt (A0A8W4F9W9) Pfam (PF01194) | Sus scrofa (pig) | |
| 14 | N (K) | DNA-directed RNA polymerase II subunit RPB11-a | polymer | 115 | 13068.0 | 1 | UniProt (F1RKE4) Pfam (PF13656) | Sus scrofa (pig) | |
| 15 | O (L) | RNA polymerase II subunit K | polymer | 58 | 7018.2 | 1 | UniProt (A0A4X1TRS6) Pfam (PF03604) | Sus scrofa (pig) | |
| 16 | P (N) | DNA | polymer | 50 | 15409.9 | 1 | synthetic construct | ||
| 17 | Q (P) | RNA | polymer | 16 | 5077.0 | 1 | synthetic construct | ||
| 18 | R (T) | DNA (38-MER) | polymer | 38 | 11728.6 | 1 | synthetic construct | ||
| 19 | S (U) | Negative elongation factor A | polymer | 527 | 55687.8 | 1 | UniProt (Q9H3P2) | Homo sapiens (human) | NELF-A,Wolf-Hirschhorn syndrome candidate 2 protein |
| 20 | T (Z) | Transcription elongation factor SPT5 | polymer | 1087 | 121145.5 | 1 | UniProt (O00267) Pfam (PF11942) Pfam (PF03439) Pfam (PF00467) | Homo sapiens (human) | hSPT5,DRB sensitivity-inducing factor 160 kDa subunit,DSIF p160,DRB sensitivity-inducing factor large subunit,DSIF large subunit,Tat-cotransactivator 1 protein,Tat-CT1 protein |
| 21 | AA, BA, CA, U, V... (I, J, L, A, B...) | ZINC ION | non-polymer | 65.4 | 8 | Chemie (ZN) | |||
| 22 | W (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence modifications
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 20 |
| Total formula weight | 898532.0 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 547.6 | |
| All* | Total formula weight | 899079.5 |
