8UCJ

CryoEM structure of Komagataella pastoris Cytochrome c oxidase (11 subunits) in complex with human VMAT2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Synaptic vesicular amine transporter	polymer	514	55749.4	1	UniProt (Q05940) In PDB	Homo sapiens (human)
2	a	Cytochrome c oxidase subunit 1	polymer	535	58857.4	1	UniProt (F2R0K8) In PDB	Komagataella pastoris
3	b	Cytochrome c oxidase subunit 2	polymer	236	26886.9	1		Komagataella pastoris
4	c	Cytochrome c oxidase subunit 3	polymer	269	30664.3	1	UniProt (F2R0J6) In PDB	Komagataella pastoris
5	d	Cytochrome c oxidase subunit 4	polymer	147	16457.8	1	UniProt (F2QT92) In PDB	Komagataella pastoris
6	e	Cytochrome c oxidase subunit 5	polymer	126	14719.6	1	UniProt (F2QVW8) In PDB	Komagataella pastoris
7	f	Cytochrome c oxidase subunit 6	polymer	100	11851.1	1	UniProt (F2QVA2) In PDB	Komagataella pastoris
8	g	Cytochrome c oxidase subunit 7	polymer	58	6573.6	1	UniProt (F2QS38) In PDB	Komagataella pastoris
9	h	Cytochrome c oxidase subunit 8	polymer	48	5456.5	1	UniProt (F2QRE4) In PDB	Komagataella pastoris
10	i	Cytochrome c oxidase subunit 9	polymer	57	6619.7	1	UniProt (A0A1G4KPQ9) In PDB	Komagataella pastoris
11	j	Cytochrome c oxidase subunit 12	polymer	75	8890.0	1	UniProt (F2QZ97) In PDB	Komagataella pastoris
12	k	Cytochrome c oxidase subunit 13	polymer	107	12518.0	1	UniProt (F2QT90) In PDB	Komagataella pastoris
13	a	COPPER (II) ION	non-polymer		63.5	1	Chemie (CU)
14	a	HEME-A	non-polymer		852.8	2	Chemie (HEA)
15	a, c, e	PHOSPHATIDYLETHANOLAMINE	non-polymer		734.0	4	Chemie (PTY)
16	b	DINUCLEAR COPPER ION	non-polymer		127.1	1	Chemie (CUA)
17	d	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
18	k	1,2-DIACYL-SN-GLYCERO-3-PHOSHOCHOLINE	non-polymer		734.0	1	Chemie (PCF)

Sequence modifications

a: 1 - 535 (UniProt: F2R0K8)

PDB	External Database	Details
Ile 4	Met 4	conflict
Ile 16	Met 16	conflict
Ile 22	Met 22	conflict
Ile 33	Met 33	conflict
Ile 37	Met 37	conflict
Ile 49	Met 49	conflict
Ile 78	Met 78	conflict
Leu 85	Thr 85	conflict
Ile 90	Met 90	conflict
Ile 121	Met 121	conflict
Ile 150	Met 150	conflict
Ile 158	Met 158	conflict
Ile 165	Met 165	conflict
Ile 168	Met 168	conflict
Ile 177	Met 177	conflict
Ile 232	Met 232	conflict
Leu 237	Thr 237	conflict
Ile 250	Met 250	conflict
Ile 256	Met 256	conflict
Ile 260	Met 260	conflict
Ile 273	Met 273	conflict
Ile 314	Met 314	conflict
Ile 320	Met 320	conflict
Ile 322	Met 322	conflict
Ile 334	Met 334	conflict
Ile 367	Met 367	conflict
Leu 401	Thr 401	conflict
Ile 402	Met 402	conflict
Ile 421	Met 421	conflict
Ile 442	Met 442	conflict
Ile 455	Met 455	conflict
Ile 462	Met 462	conflict
Ile 464	Met 464	conflict
Ile 465	Met 465	conflict
Ile 510	Met 510	conflict
Ile 515	Met 515	conflict
Leu 524	Thr 524	conflict

c: 1 - 269 (UniProt: F2R0J6)

PDB	External Database	Details
Ile 45	Met 45	conflict
Ile 55	Met 55	conflict
Ile 62	Met 62	conflict
Ile 81	Met 81	conflict
Ile 89	Met 89	conflict
Ile 101	Met 101	conflict
Ile 120	Met 120	conflict
Ile 129	Met 129	conflict
Ile 132	Met 132	conflict
Ile 143	Met 143	conflict
Ile 247	Met 247	conflict
Leu 248	Thr 248	conflict

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	255244.2
Non-Polymers*	Number of molecules	10
	Total formula weight	5631.9
All*	Total formula weight	260876.1

*Water molecules are not included.