8UCF

Thermophilic RNA Ligase from Palaeococcus pacificus K238G

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	ATP dependent DNA ligase	polymer	402	46902.0	1	UniProt (A0A075LQ94) Pfam (PF09414) Pfam (PF18330) In PDB	Palaeococcus pacificus DY20341
2	A	GLYCEROL	non-polymer		92.1	6	Chemie (GOL)
3	A	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4		water	water		18.0	224	Chemie (HOH)

Sequence modifications

A: 1 - 381 (UniProt: A0A075LQ94)

PDB	External Database	Details
Met -20	-	initiating methionine
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Val -6	-	expression tag
Pro -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag
Gly 238	Lys 238	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	46902.0
Non-Polymers*	Number of molecules	8
	Total formula weight	601.2
All*	Total formula weight	47503.2

*Water molecules are not included.