8UCE

Thermophilic RNA Ligase from Palaeococcus pacificus + AMP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	ATP dependent DNA ligase	polymer	402	46974.1	2	UniProt (A0A075LQ94) Pfam (PF09414) Pfam (PF18330)	Palaeococcus pacificus DY20341
2	C, K (A, B)	ADENOSINE MONOPHOSPHATE	non-polymer		347.2	2	Chemie (AMP)
3	D, E, F, G, H... (A, B)	GLYCEROL	non-polymer		92.1	7	Chemie (GOL)
4	I, J, N, O (A, B)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
5	P, Q (A, B)	water	water		18.0	476	Chemie (HOH)

Sequence modifications

A, B: 1 - 381 (UniProt: A0A075LQ94)

PDB	External Database	Details
Met -20	-	initiating methionine
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Val -6	-	expression tag
Pro -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	93948.3
Non-Polymers*	Number of molecules	13
	Total formula weight	1436.3
All*	Total formula weight	95384.6

*Water molecules are not included.