8UAD

Cryo-EM structure of prefusion-stabilized influenza B hemagglutinin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E (A, C, E)	Hemagglutinin HA1 chain	polymer	360	38928.7	3	UniProt (A0A2P1KSN4) Pfam (PF00509) UniProt (by SIFTS) (A0A2D1W2A5)	Influenza B virus
2	B, D, F (B, D, F)	Hemagglutinin HA2 chain	polymer	181	19675.1	3	UniProt (A0A2P1KSN4) Pfam (PF00509) UniProt (by SIFTS) (A0A2D1W645)	Influenza B virus
3	G, H, I, J, L... (G, H, I, J, L...)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	12	In PDB GlyTouCan (G15407YE)
4	K, P, U (K, P, U)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	3	In PDB GlyTouCan (G42666HT)
5	AA, V, W, X, Y... (E, A, C)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	6	Chemie (NAG)

Sequence modifications

A, C, E: -14 - 344 (UniProt: A0A2P1KSN4)

PDB	External Database	Details
Thr 209	Lys 225	engineered mutation

B, D, F: 1 - 174 (UniProt: A0A2P1KSN4)

PDB	External Database	Details
Phe 22	His 382	engineered mutation
Ala 40	Ser 400	conflict
Tyr 64	Gln 424	engineered mutation
Tyr 68	Gly 428	engineered mutation
Arg 110	Ser 470	engineered mutation
Trp 113	Glu 473	engineered mutation
Phe 114	His 474	engineered mutation
Gly 175	-	expression tag
Gly 176	-	expression tag
Ser 177	-	expression tag
Glu 178	-	expression tag
Pro 179	-	expression tag
Glu 180	-	expression tag
Ala 181	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	175811.4
Branched	Number of molecules	15
	Total formula weight	8311.7
Non-Polymers*	Number of molecules	6
	Total formula weight	1327.2
All*	Total formula weight	185450.4

*Water molecules are not included.