8U93

Crystal structure of N-acetylneuraminate lyase (NanA) from Klebsiella aerogenes (PEG bound)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	N-acetylneuraminate lyase	polymer	305	33738.6	1	UniProt (A0A0H3FJT8) Pfam (PF00701)	Klebsiella aerogenes KCTC 2190
2	B, C (A)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
3	D (A)	POTASSIUM ION	non-polymer		39.1	1	Chemie (K)
4	E (A)	2,3-DIHYDROXY-1,4-DITHIOBUTANE	non-polymer		154.3	1	Chemie (DTT)
5	F (A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
6	G, H (A)	2-(2-{2-[2-(2-METHOXY-ETHOXY)-ETHOXY]-ETHOXY}-ETHOXY)-ETHANOL	non-polymer		252.3	2	Chemie (1PG)
7	I (A)	water	water		18.0	151	Chemie (HOH)

Sequence modifications

A: 1 - 297 (UniProt: A0A0H3FJT8)

PDB	External Database	Details
Met -7	-	initiating methionine
Ala -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Ala 93	Val 93	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	33738.6
Non-Polymers*	Number of molecules	7
	Total formula weight	875.0
All*	Total formula weight	34613.6

*Water molecules are not included.