8U91

Crystal structure of N-acetylneuraminate lyase (NanA) from Klebsiella aerogenes (Apo, Orthorhombic P form)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	N-acetylneuraminate lyase	polymer	305	33669.5	6	UniProt (A0A0H3FJT8) Pfam (PF00701)	Klebsiella aerogenes KCTC 2190
2	AA, EA, FA, G, GA... (E, F, A, B, C...)	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	12	Chemie (MPD)
3	BA, CA, DA, H, HA... (E, A, F, B, C...)	CHLORIDE ION	non-polymer		35.5	21	Chemie (CL)
4	NA, OA, PA, QA, RA... (A, B, C, D, E...)	water	water		18.0	309	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F: 1 - 297 (UniProt: A0A0H3FJT8)

PDB	External Database	Details
Met -7	-	initiating methionine
Ala -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Ala 93	Val 93	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	202017.2
Non-Polymers*	Number of molecules	33
	Total formula weight	2162.6
All*	Total formula weight	204179.8

*Water molecules are not included.