8U8W

Crystal structure of N-acetylneuraminate lyase (NanA) from Klebsiella aerogenes (pyruvate and halides bound)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	N-acetylneuraminate lyase	polymer	305	33669.5	1	UniProt (A0A0H3FJT8) Pfam (PF00701) In PDB	Klebsiella aerogenes KCTC 2190
2	A	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
3	A	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
4	A	IODIDE ION	non-polymer		126.9	2	Chemie (IOD)
5	A	PYRUVIC ACID	non-polymer		88.1	1	Chemie (PYR)
6	A	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
7		water	water		18.0	275	Chemie (HOH)

Sequence modifications

A: 1 - 297 (UniProt: A0A0H3FJT8)

PDB	External Database	Details
Met -7	-	initiating methionine
Ala -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Ala 93	Val 93	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	33669.5
Non-Polymers*	Number of molecules	10
	Total formula weight	804.1
All*	Total formula weight	34473.7

*Water molecules are not included.