8U8V
Cryo-EM structure of Substrate ATP Bound in the Insertion Site (IS) of Human Mitochondrial Transcription Elongation Complex
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Transcription elongation factor, mitochondrial | polymer | 232 | 27262.4 | 2 | UniProt (Q96QE5) In PDB | Homo sapiens (human) | |
2 | E | DNA-directed RNA polymerase, mitochondrial | polymer | 1119 | 127150.1 | 1 | UniProt (O00411) Pfam (PF14700) Pfam (PF00940) In PDB | Homo sapiens (human) | |
3 | N | Non-Template Strand DNA (NT27mt) | polymer | 34 | 10483.8 | 1 | synthetic construct | ||
4 | R | RNA (RNA14mt) | polymer | 14 | 4493.7 | 1 | synthetic construct | ||
5 | T | Template Strand DNA (TS31mt) | polymer | 34 | 10419.6 | 1 | synthetic construct | ||
6 | E | DIPHOSPHOMETHYLPHOSPHONIC ACID ADENOSYL ESTER | non-polymer | 505.2 | 1 | Chemie (APC) | |||
7 | E | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence modifications
A, B: 146 - 360 (UniProt: Q96QE5)
E: 120 - 1230 (UniProt: O00411)
PDB | External Database | Details |
---|---|---|
Met 139 | - | initiating methionine |
Asp 140 | - | expression tag |
Glu 141 | - | expression tag |
Asn 142 | - | expression tag |
Ala 143 | - | expression tag |
Lys 144 | - | expression tag |
Glu 145 | - | expression tag |
Leu 361 | - | expression tag |
Glu 362 | - | expression tag |
His 363 | - | expression tag |
His 364 | - | expression tag |
His 365 | - | expression tag |
His 366 | - | expression tag |
His 367 | - | expression tag |
His 368 | - | expression tag |
His 369 | - | expression tag |
His 370 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Met 112 | - | expression tag |
Gly 113 | - | expression tag |
His 114 | - | expression tag |
His 115 | - | expression tag |
His 116 | - | expression tag |
His 117 | - | expression tag |
His 118 | - | expression tag |
His 119 | - | expression tag |
Ala 555 | Glu 555 | conflict |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 6 |
Total formula weight | 207072.0 | |
Non-Polymers* | Number of molecules | 2 |
Total formula weight | 529.5 | |
All* | Total formula weight | 207601.5 |