8U8S

Y229F/S292F Streptomyces coelicolor Laccase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Copper oxidase	polymer	351	38047.2	3	UniProt (Q9XAL8) Pfam (PF07732) Pfam (PF07731)	Streptomyces coelicolor	Laccase
2	D, E, F, GA, JA... (A, C, B)	COPPER (II) ION	non-polymer		63.5	12	Chemie (CU)
3	AA, FA, G, H, I... (B, A, C)	GLYCINE	non-polymer		75.1	15	Chemie (GLY)
4	BA, K, QA (B, A, C)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	3	Chemie (PEG)
5	CA, L, RA (B, A, C)	TRIETHYLENE GLYCOL	non-polymer		150.2	3	Chemie (PGE)
6	DA, EA, M, N, SA... (B, A, C)	TETRAETHYLENE GLYCOL	non-polymer		194.2	6	Chemie (PG4)
7	P (A)	BORIC ACID	non-polymer		61.8	1	Chemie (BO3)
8	HA, R, T (C, A, B)	HYDROXIDE ION	non-polymer		17.0	3	Chemie (OH)
9	UA, VA, WA (A, B, C)	water	water		18.0	235	Chemie (HOH)

Sequence modifications

A, B, C: 1 - 343 (UniProt: Q9XAL8)

PDB	External Database	Details
Phe 229	Tyr 229	engineered mutation
Phe 292	Ser 292	engineered mutation
Leu 344	-	expression tag
Glu 345	-	expression tag
His 346	-	expression tag
His 347	-	expression tag
His 348	-	expression tag
His 349	-	expression tag
His 350	-	expression tag
His 351	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	114141.7
Non-Polymers*	Number of molecules	43
	Total formula weight	3935.6
All*	Total formula weight	118077.4

*Water molecules are not included.