8U6X

ATP-dependent DNA ligase Lig E from Neisseria gonorrhoeae

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA ligase	polymer	279	32029.4	1	UniProt (D6HB39) UniProt (by SIFTS) (B4RQJ6)	Neisseria gonorrhoeae MS11
2	B (B)	DNA (5'-D(*TP*TP*CP*CP*GP*AP*TP*AP*GP*TP*GP*GP*GP*GP*TP*CP*GP*CP*AP*AP*T)-3')	polymer	21	6494.2	1		synthetic construct
3	C (C)	DNA (5'-D(*AP*TP*TP*GP*CP*GP*AP*CP*CP*CP*CP*AP*CP*TP*AP*TP*CP*GP*GP*AP*A)-3')	polymer	21	6392.2	1		synthetic construct
4	D (A)	ADENOSINE MONOPHOSPHATE	non-polymer		347.2	1	Chemie (AMP) PubChem (6083)
5	E, F, G (A)	THIOCYANATE ION	non-polymer		58.1	3	Chemie (SCN) PubChem (9322)
6	H, I, J (A, B, C)	water	water		18.0	42	Chemie (HOH)

Sequence modifications

A: 1 - 250 (UniProt: D6HB39)

PDB	External Database	Details
Met -22	-	initiating methionine
Ser -21	-	expression tag
Tyr -20	-	expression tag
Tyr -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
Leu -12	-	expression tag
Glu -11	-	expression tag
Ser -10	-	expression tag
Thr -9	-	expression tag
Ser -8	-	expression tag
Leu -7	-	expression tag
Tyr -6	-	expression tag
Lys -5	-	expression tag
Lys -4	-	expression tag
Ala -3	-	expression tag
Gly -2	-	expression tag
Leu -1	-	expression tag
Met 0	-	expression tag
His 251	-	expression tag
His 252	-	expression tag
His 253	-	expression tag
His 254	-	expression tag
His 255	-	expression tag
His 256	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	44915.7
Non-Polymers*	Number of molecules	4
	Total formula weight	521.5
All*	Total formula weight	45437.2

*Water molecules are not included.