8U5B

Cryo-EM structure of human claudin-4 complex with Clostridium perfringens enterotoxin C-terminal domain and sFab COP-1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Claudin-4	polymer	214	22613.9	1	UniProt (O14493) Pfam (PF00822) In PDB	Homo sapiens (human)	Clostridium perfringens enterotoxin receptor,CPE-receptor,Williams-Beuren syndrome chromosomal region 8 protein
2	B	Heat-labile enterotoxin B chain	polymer	125	14044.6	1	UniProt (P01558) In PDB	Clostridium perfringens
3	L	COP-1 sFab Light Chain	polymer	215	23258.8	1		synthetic construct
4	H	COP-1 sFab Heavy Chain	polymer	261	28064.5	1		synthetic construct
5	H	Lauryl Maltose Neopentyl Glycol	non-polymer		1005.2	1	Chemie (AV0)

Sequence modifications

A: 1 - 209 (UniProt: O14493)

PDB	External Database	Details
Gly 210	-	expression tag
Leu 211	-	expression tag
Val 212	-	expression tag
Pro 213	-	expression tag
Arg 214	-	expression tag

B: 198 - 319 (UniProt: P01558)

PDB	External Database	Details
Gly 195	-	expression tag
Ser 196	-	expression tag
Asp 197	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	87981.7
Non-Polymers*	Number of molecules	1
	Total formula weight	1005.2
All*	Total formula weight	88986.9

*Water molecules are not included.