8U3Y

SpG Cas9 with NGG PAM DNA target

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	CRISPR-associated endonuclease Cas9/Csn1	polymer	1364	158476.8	1	UniProt (Q99ZW2) Pfam (PF16593) Pfam (PF16592) Pfam (PF13395) Pfam (PF22702) Pfam (PF16595) In PDB	Streptococcus pyogenes
2	B	RNA (98-MER)	polymer	98	31702.9	1		synthetic construct
3	C	DNA (5'-D(P*CP*GP*TP*TP*TP*GP*TP*AP*CP*TP*CP*CP*AP*GP*CP*G)-3')	polymer	16	4865.2	1		synthetic construct
4	c	DNA (5'-D(P*TP*CP*TP*CP*AP*TP*CP*TP*TP*TP*AP*TP*GP*CP*GP*TP*C)-3')	polymer	17	5119.3	1		synthetic construct
5	D	DNA (5'-D(P*TP*GP*GP*AP*GP*TP*AP*CP*AP*AP*AP*CP*G)-3')	polymer	13	4024.6	1		synthetic construct
6	A, B, c	MAGNESIUM ION	non-polymer		24.3	5	Chemie (MG)
7		water	water		18.0	5	Chemie (HOH)

Sequence modifications

A: 3 - 1366 (UniProt: Q99ZW2)

PDB	External Database	Details
Leu 1135	Asp 1135	conflict
Trp 1136	Ser 1136	conflict
Lys 1218	Gly 1218	conflict
Gln 1219	Glu 1219	conflict
Gln 1335	Arg 1335	conflict
Arg 1337	Thr 1337	conflict

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	204188.8
Non-Polymers*	Number of molecules	5
	Total formula weight	121.5
All*	Total formula weight	204310.3

*Water molecules are not included.