8U3Y
SpG Cas9 with NGG PAM DNA target
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | CRISPR-associated endonuclease Cas9/Csn1 | polymer | 1364 | 158476.8 | 1 | UniProt (Q99ZW2) Pfam (PF16593) Pfam (PF16592) Pfam (PF13395) Pfam (PF22702) Pfam (PF16595) | Streptococcus pyogenes | |
| 2 | B (B) | RNA (98-MER) | polymer | 98 | 31702.9 | 1 | synthetic construct | ||
| 3 | C (C) | DNA (5'-D(P*CP*GP*TP*TP*TP*GP*TP*AP*CP*TP*CP*CP*AP*GP*CP*G)-3') | polymer | 16 | 4865.2 | 1 | synthetic construct | ||
| 4 | D (c) | DNA (5'-D(P*TP*CP*TP*CP*AP*TP*CP*TP*TP*TP*AP*TP*GP*CP*GP*TP*C)-3') | polymer | 17 | 5119.3 | 1 | synthetic construct | ||
| 5 | E (D) | DNA (5'-D(P*TP*GP*GP*AP*GP*TP*AP*CP*AP*AP*AP*CP*G)-3') | polymer | 13 | 4024.6 | 1 | synthetic construct | ||
| 6 | F, G, H, I, J (A, B, c) | MAGNESIUM ION | non-polymer | 24.3 | 5 | Chemie (MG) | |||
| 7 | K, L (A, c) | water | water | 18.0 | 5 | Chemie (HOH) |
Sequence modifications
A: 3 - 1366 (UniProt: Q99ZW2)
| PDB | External Database | Details |
|---|---|---|
| Leu 1135 | Asp 1135 | conflict |
| Trp 1136 | Ser 1136 | conflict |
| Lys 1218 | Gly 1218 | conflict |
| Gln 1219 | Glu 1219 | conflict |
| Gln 1335 | Arg 1335 | conflict |
| Arg 1337 | Thr 1337 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 204188.8 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 121.5 | |
| All* | Total formula weight | 204310.3 |
