8U3B

Cryo-EM structure of E. coli NarL-transcription activation complex at 3.2A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	DNA-directed RNA polymerase subunit alpha	polymer	329	36558.7	2	UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB	Escherichia coli	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	C	DNA-directed RNA polymerase subunit beta	polymer	1342	150820.9	1	UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) In PDB	Escherichia coli	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	D	DNA-directed RNA polymerase subunit beta'	polymer	1407	155366.8	1	UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB	Escherichia coli	RNAP subunit beta', RNA polymerase subunit beta', Transcriptase subunit beta'
4	E	DNA-directed RNA polymerase subunit omega	polymer	91	10249.5	1	UniProt (P0A800) Pfam (PF01192) In PDB	Escherichia coli	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5	F	RNA polymerase sigma factor RpoD	polymer	628	72206.3	1	UniProt (P00579) Pfam (PF03979) Pfam (PF00140) Pfam (PF04546) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) In PDB	Escherichia coli	Sigma-70
6	G, H	Nitrate/nitrite response regulator protein NarL	polymer	74	8903.6	2	UniProt (P0AF28) Pfam (PF00196) In PDB	Escherichia coli
7	1	DNA (69-MER)	polymer	69	21307.7	1		Escherichia coli
8	2	DNA (69-MER)	polymer	69	21164.5	1		Escherichia coli
9	3	RNA (5'-D(*(GTP))-R(P*AP*A)-3')	polymer	3	1118.6	1		Escherichia coli
10	D	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
11	D	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

F: 1 - 613 (UniProt: P00579)

PDB	External Database	Details
Met -14	-	expression tag
Arg -13	-	expression tag
Gly -12	-	expression tag
Ser -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
Thr -4	-	expression tag
Asp -3	-	expression tag
Gln -2	-	expression tag
Phe -1	-	expression tag
Thr 0	-	expression tag

G, H: 151 - 216 (UniProt: P0AF28)

PDB	External Database	Details
Leu 217	-	expression tag
Glu 218	-	expression tag
His 219	-	expression tag
His 220	-	expression tag
His 221	-	expression tag
His 222	-	expression tag
His 223	-	expression tag
His 224	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	11
	Total formula weight	523158.8
Non-Polymers*	Number of molecules	3
	Total formula weight	155.1
All*	Total formula weight	523313.9

*Water molecules are not included.