8U2X

Crystal Structure of NendoU (Uridylate-specific endoribonuclease, nsp15) from Betacoronavirus SARS-CoV-2 (H235A mutant)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Uridylate-specific endoribonuclease nsp15	polymer	368	41329.9	2	UniProt (P0DTD1) Pfam (PF19219) Pfam (PF19216) Pfam (PF19215)	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV SARS-CoV-2)	NendoU,Non-structural protein 15,nsp15
2	C, F (A, B)	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	2	Chemie (TRS)
3	D, E (A)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
4	G, H (A, B)	water	water		18.0	207	Chemie (HOH)

Sequence modifications

A, B: 2 - 347 (UniProt: P0DTD1)

PDB	External Database	Details
Met -20	-	initiating methionine
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
His 0	-	expression tag
Met 1	-	expression tag
Ala 235	His 6686	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	82659.7
Non-Polymers*	Number of molecules	4
	Total formula weight	315.2
All*	Total formula weight	82974.9

*Water molecules are not included.