8U2X
Crystal Structure of NendoU (Uridylate-specific endoribonuclease, nsp15) from Betacoronavirus SARS-CoV-2 (H235A mutant)
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Uridylate-specific endoribonuclease nsp15 | polymer | 368 | 41329.9 | 2 | UniProt (P0DTD1) Pfam (PF19219) Pfam (PF19216) Pfam (PF19215) In PDB | Severe acute respiratory syndrome coronavirus 2 (2019-nCoV SARS-CoV-2) | NendoU,Non-structural protein 15,nsp15 |
2 | A, B | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 122.1 | 2 | Chemie (TRS) | |||
3 | A | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) | |||
4 | water | water | 18.0 | 207 | Chemie (HOH) |
Sequence modifications
A, B: 2 - 347 (UniProt: P0DTD1)
PDB | External Database | Details |
---|---|---|
Met -20 | - | initiating methionine |
Gly -19 | - | expression tag |
Ser -18 | - | expression tag |
Ser -17 | - | expression tag |
His -16 | - | expression tag |
His -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
Ser -10 | - | expression tag |
Ser -9 | - | expression tag |
Gly -8 | - | expression tag |
Glu -7 | - | expression tag |
Asn -6 | - | expression tag |
Leu -5 | - | expression tag |
Tyr -4 | - | expression tag |
Phe -3 | - | expression tag |
Gln -2 | - | expression tag |
Gly -1 | - | expression tag |
His 0 | - | expression tag |
Met 1 | - | expression tag |
Ala 235 | His 6686 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 82659.7 | |
Non-Polymers* | Number of molecules | 4 |
Total formula weight | 315.2 | |
All* | Total formula weight | 82974.9 |