8U0F

Bacterial fluorescent protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (B, K)	Bacterial fluorescent protein alpha	polymer	164	17776.0	2	UniProt (Q1AH74)	Ceramium secundatum
2	B, D (C, L)	Bacterial fluorescent protein beta	polymer	176	18352.8	2	UniProt (Q1AH60)	Ceramium secundatum
3	E, F, H, I, J... (B, C, K, L)	PHYCOCYANOBILIN	non-polymer		588.7	8	Chemie (CYC)
4	G, L (C, L)	PHYCOUROBILIN	non-polymer		590.7	2	Chemie (PUB)
5	O, P, Q, R (B, C, K, L)	water	water		18.0	282	Chemie (HOH)

Sequence modifications

B, K: 1 - 130 (UniProt: Q1AH74)

PDB	External Database	Details
Ala 34	Ser 34	conflict
Ala 35	Gly 35	conflict
Ser 36	Ala 36	conflict
Gly 46	Ser 46	conflict
Gln 76	Thr 76	conflict
Cys 109	Gly 109	conflict
Ser 124	Ala 124	conflict
Ser 125	Ala 125	conflict

B, K: 131 - 164 (PDB: 8U0F)
C, L: 12 - 176 (UniProt: Q1AH60)

PDB	External Database	Details
Met 1	-	expression tag
Leu 2	-	expression tag
Asp 3	-	expression tag
Ala 4	-	expression tag
Phe 5	-	expression tag
Ser 6	-	expression tag
Arg 7	-	expression tag
Val 8	-	expression tag
Val 9	-	expression tag
Val 10	-	expression tag
Asn 11	-	expression tag
Ser 14	Ala 3	conflict
Ser 22	Gly 11	conflict
Gln 25	Gly 14	conflict
Thr 29	Ser 18	conflict
Asp 33	Glu 22	conflict
Ala 68	Ser 57	conflict
Thr 93	Val 82	conflict
Ala 99	Ser 88	conflict
Ser 102	Ala 91	conflict
Ser 127	Thr 116	conflict
Ser 136	Ala 125	conflict
Ile 142	Val 131	conflict
Ser 143	Thr 132	conflict
Lys 150	Thr 139	conflict
Met 151	Ile 140	conflict
Ala 152	Glu 141	conflict
Thr 153	Ile 142	conflict
Thr 154	Ala 143	conflict
Asp 155	Ala 144	conflict
Ser 162	Ala 151	conflict
Lys 171	Arg 160	conflict
Ile 176	Val 165	conflict

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	72257.6
Non-Polymers*	Number of molecules	10
	Total formula weight	5891.0
All*	Total formula weight	78148.6

*Water molecules are not included.