8TY0

Streptavidin variant S112E-K121H bound to bis-biotinylated Iron-porphyrin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Streptavidin	polymer	158	16489.8	4	UniProt (P22629) Pfam (PF01382)	Streptomyces avidinii
2	E, F (C, D)	Bis-biotinylated Iron-porphyrin	non-polymer		1177.1	2	Chemie (SIK)
3	G, H, I, J (A, B, C, D)	water	water		18.0	578	Chemie (HOH)

Sequence modifications

A, B, C, D: 14 - 159 (UniProt: P22629)

PDB	External Database	Details
Ala 2	-	expression tag
Ser 3	-	expression tag
Met 4	-	expression tag
Thr 5	-	expression tag
Gly 6	-	expression tag
Gly 7	-	expression tag
Gln 8	-	expression tag
Gln 9	-	expression tag
Met 10	-	expression tag
Gly 11	-	expression tag
Arg 12	-	expression tag
Asp 13	-	expression tag
Glu 112	Ser 136	conflict
His 121	Lys 145	conflict

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	65959.3
Non-Polymers*	Number of molecules	2
	Total formula weight	2354.3
All*	Total formula weight	68313.6

*Water molecules are not included.