8TWB
Cryo-EM structure of S. cerevisiae Ctf18-RFC-PCNA-DNA complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | 4 | Replication factor C subunit 4 | polymer | 319 | 35782.5 | 1 | UniProt (P40339) In PDB | Saccharomyces cerevisiae (brewer's yeast) | |
| 2 | 3 | Replication factor C subunit 3 | polymer | 327 | 36818.9 | 1 | UniProt (P38629) In PDB | Saccharomyces cerevisiae (brewer's yeast) | |
| 3 | 2 | Replication factor C subunit 2 | polymer | 340 | 38256.6 | 1 | UniProt (P40348) In PDB | Saccharomyces cerevisiae (brewer's yeast) | |
| 4 | 5 | Replication factor C subunit 5 | polymer | 354 | 39993.6 | 1 | UniProt (P38251) In PDB | Saccharomyces cerevisiae (brewer's yeast) | |
| 5 | 1 | Chromosome transmission fidelity protein 18 | polymer | 258 | 30182.5 | 1 | UniProt (P49956) In PDB | Saccharomyces cerevisiae (brewer's yeast) | |
| 6 | A, B, C | Proliferating cell nuclear antigen | polymer | 258 | 28944.1 | 3 | UniProt (P15873) In PDB | Saccharomyces cerevisiae (brewer's yeast) | PCNA |
| 7 | T | Template DNA | polymer | 18 | 5542.6 | 1 | synthetic construct | ||
| 8 | P | Primer DNA | polymer | 17 | 5184.4 | 1 | synthetic construct | ||
| 9 | 4, 3, 2 | PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER | non-polymer | 523.2 | 3 | Chemie (AGS) | |||
| 10 | 4, 3, 2 | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
| 11 | 5 | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 1 | Chemie (ADP) |
Sequence modifications
5: 4 - 353 (UniProt: P38251)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Ser 2 | - | expression tag |
| Leu 3 | - | expression tag |
| Asp 354 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 10 |
| Total formula weight | 278593.2 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 2069.9 | |
| All* | Total formula weight | 280663.1 |
