8TWB

Cryo-EM structure of S. cerevisiae Ctf18-RFC-PCNA-DNA complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (4)	Replication factor C subunit 4	polymer	319	35782.5	1	UniProt (P40339) Pfam (PF00004) Pfam (PF08542)	Saccharomyces cerevisiae (brewer's yeast)
2	B (3)	Replication factor C subunit 3	polymer	327	36818.9	1	UniProt (P38629) Pfam (PF00004) Pfam (PF08542)	Saccharomyces cerevisiae (brewer's yeast)
3	C (2)	Replication factor C subunit 2	polymer	340	38256.6	1	UniProt (P40348) Pfam (PF00004) Pfam (PF21960) Pfam (PF08542)	Saccharomyces cerevisiae (brewer's yeast)
4	D (5)	Replication factor C subunit 5	polymer	354	39993.6	1	UniProt (P38251) Pfam (PF22534) Pfam (PF13177) Pfam (PF21960)	Saccharomyces cerevisiae (brewer's yeast)
5	E (1)	Chromosome transmission fidelity protein 18	polymer	258	30182.5	1	UniProt (P49956)	Saccharomyces cerevisiae (brewer's yeast)
6	F, G, H (A, B, C)	Proliferating cell nuclear antigen	polymer	258	28944.1	3	UniProt (P15873) Pfam (PF00705) Pfam (PF02747)	Saccharomyces cerevisiae (brewer's yeast)	PCNA
7	I (T)	Template DNA	polymer	18	5542.6	1		synthetic construct
8	J (P)	Primer DNA	polymer	17	5184.4	1		synthetic construct
9	K, M, O (4, 3, 2)	PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER	non-polymer		523.2	3	Chemie (AGS)
10	L, N, P (4, 3, 2)	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
11	Q (5)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)

Sequence modifications

5: 4 - 353 (UniProt: P38251)

PDB	External Database	Details
Met 1	-	initiating methionine
Ser 2	-	expression tag
Leu 3	-	expression tag
Asp 354	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	278593.2
Non-Polymers*	Number of molecules	7
	Total formula weight	2069.9
All*	Total formula weight	280663.1

*Water molecules are not included.