8TTW
Cryo-EM structure of BG505 SOSIP.664 HIV-1 Env trimer in complex with temsavir, 8ANC195, and 10-1074
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, E, I (A, E, I) | Envelope glycoprotein gp160 | polymer | 481 | 54064.3 | 3 | UniProt (Q2N0S6) Pfam (PF00516) | Human immunodeficiency virus 1 | Env polyprotein |
| 2 | B, F, J (B, F, J) | BG505 SOSIP.664 transmembrane protein gp41 | polymer | 153 | 17146.5 | 3 | UniProt (Q2N0S5) Pfam (PF00517) | Human immunodeficiency virus 1 | |
| 3 | C, G, K (C, G, K) | Antibody 8anc195 heavy chain | polymer | 238 | 25324.4 | 3 | Homo sapiens (human) | ||
| 4 | D, H, L (D, H, L) | Antibody 8anc195 light chain | polymer | 215 | 23460.0 | 3 | Homo sapiens (human) | ||
| 5 | M, O, Q (M, O, Q) | Antibody 10-1074 heavy chain | polymer | 238 | 25661.7 | 3 | Homo sapiens (human) | ||
| 6 | N, P, R (N, P, R) | Antibody 10-1074 light chain | polymer | 214 | 23180.8 | 3 | Homo sapiens (human) | ||
| 7 | AA, BB, CB, DA, EA... (a, 1, 2, d, e...) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 33 | In PDB GlyTouCan (G42666HT) | |||
| 8 | BA, NA, ZA (b, n, z) | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1721.5 | 3 | In PDB GlyTouCan (G40702WU) | |||
| 9 | AB, CA, OA (0, c, o) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 3 | In PDB GlyTouCan (G22768VO) | |||
| 10 | DB, FA, RA (3, f, r) | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1559.4 | 3 | In PDB GlyTouCan (G68668TB) | |||
| 11 | AC, BC, DC, EC, FC... (I, J, K, A, B...) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 21 | Chemie (NAG) | |||
| 12 | CC, MB, UB (I, A, E) | 1-[4-(benzenecarbonyl)piperazin-1-yl]-2-[4-methoxy-7-(3-methyl-1H-1,2,4-triazol-1-yl)-1H-pyrrolo[2,3-c]pyridin-3-yl]ethane-1,2-dione | non-polymer | 473.5 | 3 | Chemie (83J) |
Sequence modifications
A, E, I: 31 - 513 (UniProt: Q2N0S6)
B, F, J: 512 - 664 (UniProt: Q2N0S5)
| PDB | External Database | Details |
|---|---|---|
| Asn 332 | Thr 330 | engineered mutation |
| Cys 501 | Ala 498 | engineered mutation |
| Arg 509 | Glu 506 | engineered mutation |
| Arg 510 | Lys 507 | engineered mutation |
| Arg 512 | Ala 509 | engineered mutation |
| Arg 513 | Val 510 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Pro 559 | Ile 556 | engineered mutation |
| Cys 605 | Thr 602 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 18 |
| Total formula weight | 506513.0 | |
| Branched | Number of molecules | 42 |
| Total formula weight | 26580.4 | |
| Non-Polymers* | Number of molecules | 24 |
| Total formula weight | 6065.8 | |
| All* | Total formula weight | 539159.2 |
