8TSX

Pseudomonas fluorescens G150T isocyanide hydratase at 100 K

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Isonitrile hydratase InhA	polymer	231	24224.7	2	UniProt (Q4K977) Pfam (PF01965)	Pseudomonas fluorescens	Isocyanide hydratase
2	C, D, E, F, G (A, B)	CHLORIDE ION	non-polymer		35.5	5	Chemie (CL)
3	H, I (A, B)	water	water		18.0	447	Chemie (HOH)

Sequence modifications

A, B: 1 - 228 (UniProt: Q4K977)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Thr 150	Gly 150	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	48449.4
Non-Polymers*	Number of molecules	5
	Total formula weight	177.3
All*	Total formula weight	48626.7

*Water molecules are not included.