8TSH

S. thermodepolymerans KpsMT(E151Q)-KpsE in complex with ATP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	ABC transporter ATP-binding protein	polymer	234	26223.7	2	UniProt (A0A2S5T4B3) Pfam (PF00005)	Caldimonas thermodepolymerans
2	C, D (C, D)	Transport permease protein	polymer	274	30801.0	2	UniProt (A0A2S5T447) Pfam (PF01061)	Caldimonas thermodepolymerans
3	E, F, G, H, I... (E, F, G, H, I...)	Capsular biosynthesis protein	polymer	390	44057.3	8	UniProt (A0A2S5T4A0)	Caldimonas thermodepolymerans
4	M, O (A, B)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (ATP)
5	N, P (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

A, B: 1 - 226 (UniProt: A0A2S5T4B3)

PDB	External Database	Details
Gln 151	Glu 151	engineered mutation
Asp 227	-	expression tag
Tyr 228	-	expression tag
Lys 229	-	expression tag
Asp 230	-	expression tag
Asp 231	-	expression tag
Asp 232	-	expression tag
Asp 233	-	expression tag
Lys 234	-	expression tag

C, D: 4 - 271 (UniProt: A0A2S5T447)

PDB	External Database	Details
Met -2	-	initiating methionine
Gly -1	-	expression tag
Lys 0	-	expression tag
Ile 1	-	expression tag
His 2	-	expression tag
Leu 3	-	expression tag

E, F, G, H, I, J, K, L: 3 - 371 (UniProt: A0A2S5T4A0)

PDB	External Database	Details
Met -2	-	initiating methionine
Gly -1	-	expression tag
Lys 0	-	expression tag
Ile 1	-	expression tag
His 2	-	expression tag
Cys 77	Leu 76	conflict
Cys 138	Ser 137	conflict
Gly 372	-	expression tag
Ser 373	-	expression tag
Gly 374	-	expression tag
Ser 375	-	expression tag
Gly 376	-	expression tag
Ser 377	-	expression tag
His 378	-	expression tag
His 379	-	expression tag
His 380	-	expression tag
His 381	-	expression tag
His 382	-	expression tag
His 383	-	expression tag
His 384	-	expression tag
His 385	-	expression tag
His 386	-	expression tag
His 387	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	466507.5
Non-Polymers*	Number of molecules	4
	Total formula weight	1063.0
All*	Total formula weight	467570.5

*Water molecules are not included.