8TS1

Cryo-EM structure of human MRS2 with Mg2+

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E (A, B, C, D, E)	Magnesium transporter MRS2 homolog, mitochondrial, Soluble cytochrome b562 fusion protein	polymer	486	55471.7	5	UniProt (Q9HD23) UniProt (P0ABE7) Pfam (PF22099) Pfam (PF07361)	Homo sapiens (human)
2	F, G, H, I, J... (A, B, C, D, E)	MAGNESIUM ION	non-polymer		24.3	7	Chemie (MG)

Sequence modifications

A, B, C, D, E: 62 - 431 (UniProt: Q9HD23)

PDB	External Database	Details
Met 61	-	initiating methionine

A, B, C, D, E: 432 - 536 (UniProt: P0ABE7)

PDB	External Database	Details
Trp 438	Met 29	conflict
Ile 533	His 124	conflict
Leu 537	-	expression tag
Ser 538	-	expression tag
Asn 539	-	expression tag
Ser 540	-	expression tag
Leu 541	-	expression tag
Glu 542	-	expression tag
Val 543	-	expression tag
Leu 544	-	expression tag
Phe 545	-	expression tag
Gln 546	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	277358.5
Non-Polymers*	Number of molecules	7
	Total formula weight	170.1
All*	Total formula weight	277528.6

*Water molecules are not included.