8TQF

Crystal structure of Soybean SHMT8 in complex with PLP-glycine and diglutamylated 5-formyltetrahydrofolate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Serine hydroxymethyltransferase	polymer	492	54141.4	4	UniProt (A0A0R0IK90) Pfam (PF00464) In PDB	Glycine max (soybean)
2	A, B, C, D	N-GLYCINE-[3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-YL-METHANE]	non-polymer		306.2	4	Chemie (PLG)
3	A, B, C, D	1,2-ETHANEDIOL	non-polymer		62.1	13	Chemie (EDO)
4	A, B, C, D	N-[4-({[(6R)-2-amino-5-formyl-4-hydroxy-5,6,7,8-tetrahydropteridin-6-yl]methyl}amino)benzoyl]-L-gamma-glutamyl-L-glutamic acid	non-polymer		602.6	4	Chemie (S8R)
5		water	water		18.0	1478	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 471 (UniProt: A0A0R0IK90)

PDB	External Database	Details
Met -20	-	initiating methionine
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	216565.4
Non-Polymers*	Number of molecules	21
	Total formula weight	4441.9
All*	Total formula weight	221007.4

*Water molecules are not included.