8TPO
nhTMEM16 R432A mutant in lipid nanodiscs with MSP1E3 scaffold protein in the presence of Ca2+
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (B, A) | Lipid scramblase nhTMEM16 | polymer | 737 | 83207.9 | 2 | UniProt (C7Z7K1) Pfam (PF20877) Pfam (PF04547) | Fusarium vanettenii | |
| 2 | C, D, H, I (B, A) | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) | |||
| 3 | E, F, G, J (B, A) | (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate | non-polymer | 749.0 | 4 | Chemie (PGW) |
Sequence modifications
B, A: 2 - 735 (UniProt: C7Z7K1)
| PDB | External Database | Details |
|---|---|---|
| Gly 0 | - | expression tag |
| Pro 1 | - | expression tag |
| Ala 432 | Arg 432 | engineered mutation |
| Ala 736 | - | cloning artifact |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 166415.9 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 3156.3 | |
| All* | Total formula weight | 169572.2 |
