8TOM

Escherichia coli RNA polymerase closed complex intermediate at the lambda PR promoter

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	G, H, M	DNA-directed RNA polymerase subunit alpha	polymer	329	36558.7	3	UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB	Escherichia coli (strain K12)	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	I	DNA-directed RNA polymerase subunit beta	polymer	1342	150820.9	1	UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) In PDB	Escherichia coli (strain K12)	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	J	DNA-directed RNA polymerase subunit beta'	polymer	1407	155366.8	1	UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB	Escherichia coli (strain K12)	RNAP subunit beta', RNA polymerase subunit beta', Transcriptase subunit beta'
4	K	DNA-directed RNA polymerase subunit omega	polymer	91	10249.5	1	UniProt (P0A800) Pfam (PF01192) In PDB	Escherichia coli (strain K12)	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5	L	RNA polymerase sigma factor RpoD	polymer	613	70352.2	1	UniProt (Q0P6L9) Pfam (PF03979) Pfam (PF00140) Pfam (PF04546) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) In PDB	Escherichia coli (strain K12)	Sigma-70
6	O	Nontemplate strand of lamdba PR promoter DNA	polymer	90	27881.8	1		Escherichia phage Lambda
7	P	Template strand of lamdba PR promoter DNA	polymer	90	27633.7	1		Escherichia phage Lambda
8	I, J, L	CHAPSO	non-polymer		631.9	4	Chemie (1N7)
9	J	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
10	J	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	551981.0
Non-Polymers*	Number of molecules	7
	Total formula weight	2682.7
All*	Total formula weight	554663.7

*Water molecules are not included.