8TMP

Cryo-EM structure of magnesium depleted CorA in complex with conformation-specific synthetic antibody C18, State MGD-1B

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (L)	sAB C18 Light Chain	polymer	160	17167.1	1		Homo sapiens
2	B (H)	sAB C18 Heavy Chain	polymer	160	16995.8	1		Homo sapiens
3	C, D, E, F, G (A, B, C, D, E)	Cobalt/magnesium transport protein CorA	polymer	373	44067.8	5	UniProt (Q9WZ31) Pfam (PF01544)	Thermotoga maritima
4	H, I (A, D)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

A, B, C, D, E: 1 - 351 (UniProt: Q9WZ31)

PDB	External Database	Details
Met -21	-	initiating methionine
Gly -20	-	expression tag
Ser -19	-	expression tag
Ser -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
Gly -9	-	expression tag
Arg -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	254501.9
Non-Polymers*	Number of molecules	2
	Total formula weight	48.6
All*	Total formula weight	254550.6

*Water molecules are not included.