8TL3

CRYO-EM STRUCTURE OF HIV-1 BG505DS-SOSIP.664 ENV TRIMER BOUND TO DJ85-d.01 FAB

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E	BG505 DS-SOSIP glycoprotein gp120	polymer	481	54086.3	3	UniProt (Q2N0S5) Pfam (PF00516) In PDB	Human immunodeficiency virus 1
2	B, D, F	BG505 DS-SOSIP glycoprotein gp41	polymer	153	17146.5	3	UniProt (Q2N0S5) Pfam (PF00517) In PDB	Human immunodeficiency virus 1
3	G, I, K	DJ85-d.01 FAB HEAVY CHAIN	polymer	237	25400.3	3		Homo sapiens
4	H, J, L	DJ85-d.01 FAB LIGHT CHAIN	polymer	214	23575.0	3		Homo sapiens
5	Z, b, d, e, f...	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	24	In PDB GlyTouCan (G42666HT)
6	Y, j, Q	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	3	In PDB GlyTouCan (G42227JK)
7	E, A, C	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	18	Chemie (NAG)

Sequence modifications

A, C, E: 31 - 511 (UniProt: Q2N0S5)

PDB	External Database	Details
Cys 201	Ile 200	engineered mutation
Asn 332	Thr 330	engineered mutation
Cys 433	Ala 430	engineered mutation
Cys 501	Ala 498	engineered mutation
Arg 509	Glu 506	engineered mutation
Arg 510	Lys 507	engineered mutation
Arg 512	-	insertion
Arg 513	-	insertion

B, D, F: 512 - 664 (UniProt: Q2N0S5)

PDB	External Database	Details
Pro 559	Ile 556	engineered mutation
Cys 605	Thr 602	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	360624.4
Branched	Number of molecules	27
	Total formula weight	12918.1
Non-Polymers*	Number of molecules	18
	Total formula weight	3981.7
All*	Total formula weight	377524.2

*Water molecules are not included.