8TKC
CRYO-EM STRUCTURE OF HIV-1 BG505DS-SOSIP.664 ENV TRIMER BOUND TO DJ85-b.01 FAB
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C, E | BG505 DS-SOSIP Surface protein gp120 | polymer | 481 | 54086.3 | 3 | UniProt (Q2N0S5) Pfam (PF00516) In PDB | Human immunodeficiency virus 1 | Env polyprotein |
| 2 | B, D, F | BG505 DS-SOSIP Transmembrane protein gp41 | polymer | 153 | 17146.5 | 3 | UniProt (Q2N0S5) Pfam (PF00517) In PDB | Human immunodeficiency virus 1 | Env polyprotein |
| 3 | G, I, K | DJ85-b.01 FAB HEAVY CHAIN | polymer | 240 | 25501.3 | 3 | Homo sapiens | ||
| 4 | H, J, L | DJ85-b.01 FAB LIGHT CHAIN | polymer | 214 | 23429.9 | 3 | Homo sapiens | ||
| 5 | Y, Z, d, e, f... | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 24 | In PDB GlyTouCan (G42666HT) | |||
| 6 | j, O, X | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 3 | In PDB GlyTouCan (G15407YE) | |||
| 7 | b, m, R | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 3 | In PDB GlyTouCan (G42227JK) | |||
| 8 | E, A, C | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 15 | Chemie (NAG) |
Sequence modifications
A, C, E: 31 - 511 (UniProt: Q2N0S5)
B, D, F: 512 - 664 (UniProt: Q2N0S5)
| PDB | External Database | Details |
|---|---|---|
| Cys 201 | Ile 200 | engineered mutation |
| Asn 332 | Thr 330 | engineered mutation |
| Cys 433 | Ala 430 | engineered mutation |
| Cys 501 | Ala 498 | engineered mutation |
| Arg 509 | Glu 506 | engineered mutation |
| Arg 510 | Lys 507 | engineered mutation |
| Arg 512 | - | insertion |
| Arg 513 | - | insertion |
| PDB | External Database | Details |
|---|---|---|
| Pro 559 | Ile 556 | engineered mutation |
| Cys 605 | Thr 602 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 360492.0 | |
| Branched | Number of molecules | 30 |
| Total formula weight | 14677.7 | |
| Non-Polymers* | Number of molecules | 15 |
| Total formula weight | 3318.1 | |
| All* | Total formula weight | 378487.9 |
