8TJS

CRYO-EM STRUCTURE OF HIV-1 BG505DS-SOSIP.664 ENV TRIMER BOUND TO GPZ6-a.01 FAB

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	G, N, O	Envelope glycoprotein gp160	polymer	480	53987.2	3	UniProt (Q2N0S6) In PDB	Human immunodeficiency virus 1	Env polyprotein
2	B, H, I	Envelope glycoprotein gp41	polymer	153	17146.5	3	UniProt (Q2N0S6) In PDB	Human immunodeficiency virus 1	Env polyprotein
3	Q, P, R	Antibody GPZ6-a.01 Heavy Chain	polymer	126	13994.7	3		Macaca
4	T, S, U	Antibody GPZ6-a.01 Light Chain	polymer	107	11552.8	3		Macaca
5	A, E, F	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1073.0	3	In PDB GlyTouCan (G56014GC)
6	C, D, J, L, K...	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	6	In PDB GlyTouCan (G42666HT)
7	G, H, O, B, N...	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	48	Chemie (NAG)

Sequence modifications

G, N, O: 31 - 514 (UniProt: Q2N0S6)

PDB	External Database	Details
Cys 210	Ile 200	conflict
Asn 341	Thr 330	conflict
Cys 442	Ala 430	conflict
Cys 510	Ala 498	conflict
Gly 515	-	expression tag
Arg 516	-	expression tag
Arg 517	-	expression tag
Arg 518	-	expression tag
Arg 519	-	expression tag
Arg 520	-	expression tag
Arg 521	-	expression tag

B, H, I: 512 - 664 (UniProt: Q2N0S6)

PDB	External Database	Details
Pro 559	Ile 556	engineered mutation
Cys 605	Thr 602	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	290043.5
Branched	Number of molecules	9
	Total formula weight	5765.3
Non-Polymers*	Number of molecules	48
	Total formula weight	10618.0
All*	Total formula weight	306426.8

*Water molecules are not included.