8TJS
CRYO-EM STRUCTURE OF HIV-1 BG505DS-SOSIP.664 ENV TRIMER BOUND TO GPZ6-a.01 FAB
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C, E (G, N, O) | Envelope glycoprotein gp160 | polymer | 480 | 53987.2 | 3 | UniProt (Q2N0S6) Pfam (PF00516) | Human immunodeficiency virus 1 | Env polyprotein |
| 2 | B, D, F (B, H, I) | Envelope glycoprotein gp41 | polymer | 153 | 17146.5 | 3 | UniProt (Q2N0S6) Pfam (PF00517) | Human immunodeficiency virus 1 | Env polyprotein |
| 3 | G, I, K (Q, P, R) | Antibody GPZ6-a.01 Heavy Chain | polymer | 126 | 13994.7 | 3 | Macaca | ||
| 4 | H, J, L (T, S, U) | Antibody GPZ6-a.01 Light Chain | polymer | 107 | 11552.8 | 3 | Macaca | ||
| 5 | M, P, S (A, E, F) | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1073.0 | 3 | In PDB GlyTouCan (G56014GC) | |||
| 6 | N, O, Q, R, T... (C, D, J, L, K...) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 6 | In PDB GlyTouCan (G42666HT) | |||
| 7 | AA, AB, BA, BB, CA... (G, H, O, B, N...) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 48 | Chemie (NAG) |
Sequence modifications
G, N, O: 31 - 514 (UniProt: Q2N0S6)
B, H, I: 512 - 664 (UniProt: Q2N0S6)
| PDB | External Database | Details |
|---|---|---|
| Cys 210 | Ile 200 | conflict |
| Asn 341 | Thr 330 | conflict |
| Cys 442 | Ala 430 | conflict |
| Cys 510 | Ala 498 | conflict |
| Gly 515 | - | expression tag |
| Arg 516 | - | expression tag |
| Arg 517 | - | expression tag |
| Arg 518 | - | expression tag |
| Arg 519 | - | expression tag |
| Arg 520 | - | expression tag |
| Arg 521 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Pro 559 | Ile 556 | engineered mutation |
| Cys 605 | Thr 602 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 290043.5 | |
| Branched | Number of molecules | 9 |
| Total formula weight | 5765.3 | |
| Non-Polymers* | Number of molecules | 48 |
| Total formula weight | 10618.0 | |
| All* | Total formula weight | 306426.8 |
