8TJR

CRYO-EM STRUCTURE OF HIV-1 BG505DS-SOSIP.664 ENV TRIMER BOUND TO HERH-a.01 FAB

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, G (J, H)	Antibody HERH-a.01 Heavy Chain	polymer	114	12659.1	2		Macaca
2	B, H (K, L)	Antibody HERH-a.01 Light Chain	polymer	112	12193.7	2		Macaca
3	C, E, I (A, B, C)	Envelope glycoprotein gp160	polymer	475	53143.2	3	UniProt (Q2N0S6)	Human immunodeficiency virus 1	Env polyprotein
4	D, F, J (D, E, F)	Envelope glycoprotein gp41	polymer	153	17146.5	3	UniProt (Q2N0S6)	Human immunodeficiency virus 1	Env polyprotein
5	K, N, Q (P, U, X)	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1073.0	3	In PDB GlyTouCan (G56014GC)
6	L, M, O, P, R... (Q, R, V, W, Y...)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	6	In PDB GlyTouCan (G42666HT)
7	AA, AB, BA, BB, CA... (A, C, D, B, F...)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	48	Chemie (NAG)

Sequence modifications

A, B, C: 31 - 516 (UniProt: Q2N0S6)

PDB	External Database	Details
Cys 210	Ile 200	conflict
Asn 341	Thr 330	conflict
Cys 442	Ala 430	conflict
Cys 510	Ala 498	conflict

D, E, F: 512 - 664 (UniProt: Q2N0S6)

PDB	External Database	Details
Pro 559	Ile 556	engineered mutation
Cys 605	Thr 602	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	260574.5
Branched	Number of molecules	9
	Total formula weight	5765.3
Non-Polymers*	Number of molecules	48
	Total formula weight	10618.0
All*	Total formula weight	276957.8

*Water molecules are not included.