8TJN

Crosslinked 6-deoxyerythronolide B synthase (DEBS) Module 1 in complex with antibody fragment 1B2: Crosslinked State 1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B, A	EryAI,6-deoxyerythronolide-B synthase EryA3, modules 5 and 6	polymer	1784	188891.2	2	UniProt (Q5UNP6) UniProt (Q03133) In PDB	Saccharopolyspora erythraea	DEBS 3,6-deoxyerythronolide B synthase III,Erythronolide synthase,ORF C
2	C, E	Antibody Fragment 1B2, Heavy Chain	polymer	249	26447.6	2		Homo sapiens (human)
3	D, F	Antibody Fragment 1B2, Light Chain	polymer	236	25715.8	2		Homo sapiens (human)

Sequence modifications

B, A: 32 - 1490 (UniProt: Q5UNP6)

PDB	External Database	Details
Met 1	-	expression tag
Ala 2	-	expression tag
Ser 3	-	expression tag
Thr 4	-	expression tag
Asp 5	-	expression tag
Ser 6	-	expression tag
Glu 7	-	expression tag
Lys 8	-	expression tag
Val 9	-	expression tag
Ala 10	-	expression tag
Glu 11	-	expression tag
Tyr 12	-	expression tag
Leu 13	-	expression tag
Arg 14	-	expression tag
Arg 15	-	expression tag
Ala 16	-	expression tag
Thr 17	-	expression tag
Leu 18	-	expression tag
Asp 19	-	expression tag
Leu 20	-	expression tag
Arg 21	-	expression tag
Ala 22	-	expression tag
Ala 23	-	expression tag
Arg 24	-	expression tag
Gln 25	-	expression tag
Arg 26	-	expression tag
Ile 27	-	expression tag
Arg 28	-	expression tag
Glu 29	-	expression tag
Leu 30	-	expression tag
Glu 31	-	expression tag
Thr 1486	Ala 2011	conflict
Ser 1487	Ala 2012	conflict

B, A: 1491 - 1767 (UniProt: Q03133)

PDB	External Database	Details
Ser 1768	-	expression tag
Ser 1769	-	expression tag
Val 1770	-	expression tag
Asp 1771	-	expression tag
Lys 1772	-	expression tag
Leu 1773	-	expression tag
Ala 1774	-	expression tag
Ala 1775	-	expression tag
Ala 1776	-	expression tag
Leu 1777	-	expression tag
Glu 1778	-	expression tag
His 1779	-	expression tag
His 1780	-	expression tag
His 1781	-	expression tag
His 1782	-	expression tag
His 1783	-	expression tag
His 1784	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	482109.3
All*	Total formula weight	482109.3

*Water molecules are not included.